TCR beta antibody (FITC)

Catalog No. ABIN6657906

Product Details anti-TCR beta Antibody (FITC)

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TCR beta antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with TCRbeta from other sources has not been tested

Purification: Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta. . Anti-TCRbeta is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Immunogen:

Immunogen: Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.

Clone: H57-597

Isotype: IgG

Labeling Ratio: 2-8

Application Details

Application Notes:

Application Note: Anti-TCRbeta is useful for Immunofluorescence, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Target Details for TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Alternative Name: TCRbeta (TCR beta Products)

Synonyms: TCRbeta antibody, Tib antibody, T cell receptor beta chain antibody, Tcrb antibody

Background:

Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

Background: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.

Gene Name: Tcrb

Gene ID: 21577