TCR beta antibody (PE)

Catalog No. ABIN6657907

Product Details anti-TCR beta Antibody (PE)

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TCR beta antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity with TCRbeta from other sources has not been tested

Purification: Phycoerythrin conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta. . Anti-TCRbeta is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Immunogen:

Immunogen: Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with Affinity purified TCR from mouse DO-11.10 cells.

Immunogen Type: Other

Clone: H57-597

Isotype: IgG

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-TCRbeta is useful for Immunoprecipitation, Immunohistochemistry, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Target Details for TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Alternative Name: TCRbeta (TCR beta Products)

Synonyms: TCRbeta antibody, Tib antibody, T cell receptor beta chain antibody, Tcrb antibody

Background:

Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

Background: T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Gene Name: Tcrb

Gene ID: 21577