Tryptophan Hydroxylase 1 antibody

Catalog No. ABIN6658049

Supplier Product No.: 600-601-c96

This anti-Tryptophan Hydroxylase 1 antibody is a Sheep Polyclonal antibody detecting Tryptophan Hydroxylase 1 in WB and IHC. Suitable for Rabbit.

Quick Overview for Tryptophan Hydroxylase 1 antibody (ABIN6658049)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Supplier Product No.: 600-601-c96

Supplier: Rockland

Purpose: Tryptophan Hydroxylase Antibody

Cross-Reactivity (Details): Anti-Tryptophan Hydroxylase antibody is directed against Tryptophan 5-hydroxylase 1.

Purification: Tryptophan Hydroxylase antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Tryptophan Hydroxylase Antibody was produced in sheep by repeated immunizations with recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Tryptophan Hydroxylase (Sheep) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protein in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: Tryptophan Hydroxylase

Background:

Synonyms: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1

Background: Tryptophan Hydroxylase antibody detects Tryptophan Hydroxylase. TPH catalyzes the first step in the biosynthesis of serotonin and melatonin. Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Anti-Tryptophan Hydroxylase antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: TPH1

Gene ID: 100101558

NCBI Accession: NP_001093425

UniProt: P17290

Pathways: Feeding Behaviour