5HT1B Receptor antibody

Catalog No. ABIN6658050

Supplier Product No.: 200-401-i11

This Rabbit Polyclonal antibody specifically detects 5HT1B Receptor in WB. It exhibits reactivity toward Rat.

Quick Overview for 5HT1B Receptor antibody (ABIN6658050)

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5HT1B Receptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-5HT1B Receptor Antibody

Supplier Product No.: 200-401-i11

Supplier: Rockland

Purpose: Serotonin Receptor 1B Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Serotonin Receptor 1B from human, mouse and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Serotonin Receptor 1B Antibody was purified by Protein G chromatography.

Immunogen: Serotonin Receptor 1B Antibody was produced from whole rabbit serum prepared by repeated immunizations with a mixture of synthetic peptides corresponding to two internal amino acid sequences in rat 5-HT1BR protein.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1-2 μg/mL

Comment:

Anti-Serotonin Receptor 1B antibody is tested for use in WB and suitable in IF/ICC. Expect a band approximately 40kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for 5HT1B Receptor

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Alternative Name: Serotonin Receptor 1B

Background:

Synonyms: 5ht1b

Background: Anti-Serotonin Receptor 1B antibody detects rat Serotonin Receptor 1B. Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. Molecular cloning studies have identified 5 receptors belonging to the 5-HT1 subtypes. These receptors are negatively coupled to adenylyl cyclase. Radioligand binding studies suggest the presence of 5-HT1B (formerly known as 5-HT1DbR) sites in substantia nigra, dorsal subiculum, globus pallidus, and superior collicum. The in situ hybridization studies show abundance of 5-HT1BR mRNA in CA1 pyramidal cells of hippocampus, Purkinje cells of cerebellum, olfactory tubercle, subthalamic nucleus and somewhat lower levels in the caudate-putamen, cortical areas, thalamic and hypothalamic nuclei. Anti-Serotonin Receptor 1B antibody is ideal for investigators involved in neurobiology.

Gene Name: Htr1b

Gene ID: 25075

NCBI Accession: NP_071561

UniProt: P28564

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins