5HT1B Receptor antibody (Extracellular, N-Term)

Catalog No. ABIN7887147

The Rabbit Polyclonal anti-5HT1B Receptor antibody (ABIN7887147) specifically detects 5HT1B Receptor in WB, IHC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for 5HT1B Receptor antibody (Extracellular, N-Term) (ABIN7887147)

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5HT1B Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-5HT1B Receptor Antibody

Binding Specificity: AA 33-49, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 15,17 amino acid residues identical, mouse - 14

Characteristics: Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (ABIN7043616, ABIN7045280 and ABIN7045281) is a highly specific antibody directed against an epitope of the human 5-Hydroxytryptamine receptor 1B. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT1B receptor from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236784)

Blocking Peptide: (ABIN7236784)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for 5HT1B Receptor

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Alternative Name: HTR1B

Background:

Synonyms: 5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2

Description: Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.The 5-hydroxytryptamine receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric diseases, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1.Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1.

Gene ID: 3351

NCBI Accession: NM_000863

UniProt: P28222

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins