NEFM antibody (C-Term)

Catalog No. ABIN6658051

Supplier Product No.: 212-901-d84

Product Details anti-NEFM Antibody

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Binding Specificity: C-Term

Reactivity: Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 212-901-d84

Supplier: Rockland

Purpose: Neurofilament M Antibody

Cross-Reactivity (Details): Anti-NF-M antibody is directed against NF-M protein.

Purification: The antibody is a total IgY fraction.

Immunogen: Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:5000

Other: User Optimized

Comment:

Suggested Applications: IHC
Anti- Neurofilament M antibody is tested for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NEFM antibody (ABIN6658051)

Procacci, Hastings, Aziz, Christiansen, Zhao, DeAngeli, LeBlanc, Notterpek, Valdez, Gould: "Kir4.1 is specifically expressed and active in non-myelinating Schwann cells." in: Glia, Vol. 71, Issue 4, pp. 926-944, (2023) (PubMed).

Perez-Gonzalez, Provost, Rousse, Piovesana, Benzina, Darabid, Lamoureux, Wang, Arbour, Robitaille: "Functional adaptation of glial cells at neuromuscular junctions in response to injury." in: Glia, Vol. 70, Issue 9, pp. 1605-1629, (2022) (PubMed).

Martineau, Arbour, Vallée, Robitaille: "Properties of Glial Cell at the Neuromuscular Junction Are Incompatible with Synaptic Repair in the SOD1G37R ALS Mouse Model." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 40, Issue 40, pp. 7759-7777, (2021) (PubMed).

Darabid, St-Pierre-See, Robitaille: "Purinergic-Dependent Glial Regulation of Synaptic Plasticity of Competing Terminals and Synapse Elimination at the Neuromuscular Junction." in: Cell reports, Vol. 25, Issue 8, pp. 2070-2082.e6, (2019) (PubMed).

Martineau, Di Polo, Vande Velde, Robitaille: "Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS." in: eLife, Vol. 7, (2019) (PubMed).

Darabid, Arbour, Robitaille: "Glial cells decipher synaptic competition at the mammalian neuromuscular junction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 4, pp. 1297-313, (2013) (PubMed).

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament M (NEFM Products)

Background:

Synonyms: chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Neurofilament M Antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: NEFM

Gene ID: 24588

UniProt: P12839

Pathways: Brown Fat Cell Differentiation