MBP antibody

Catalog No. ABIN6658054

Supplier Product No.: 200-901-d81

The Chicken Polyclonal anti-MBP antibody has been validated for WB, IF and FM. It is suitable to detect MBP in samples from Rat.

Quick Overview for MBP antibody (ABIN6658054)

Target: MBP (Myelin Basic Protein (MBP))

Reactivity: Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This MBP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-MBP Antibody

Supplier Product No.: 200-901-d81

Supplier: Rockland

Purpose: Myelin Basic Protein (MBP) Antibody

Cross-Reactivity (Details): Anti-MBP antibody is directed against Myelin basic protein.

Purification: The antibody is a total IgY fraction.

Immunogen: Anti-Myelin basic protein Antibody was produced in chicken by repeated immunizations with three peptide sequences conserved in higher vertebrate MBP proteins.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:5000

Comment:

Anti-Myelin Basic Protein antibody is tested for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Background:

Synonyms: Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin basic protein, MBP, Myelin basic protein S, MBP S antibody

Background: Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: MDV1

Gene ID: 24547

NCBI Accession: NP_001020465

UniProt: P02688