SCN2A antibody (C-Term)

Catalog No. ABIN6658123

Product Details anti-SCN2A Antibody

Target: SCN2A (Sodium Channel, Voltage-Gated, Type II, alpha Subunit (SCN2A))

Binding Specificity: C-Term

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCN2A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Nav1.2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Nav1.2 from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Nav1.2 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: Nav1.2 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to the cytoplasmic C-terminus region of rat Nav1.2.

Immunogen Type: Recombinant Protein

Clone: S69-3

Isotype: IgG2a

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-Nav1.2 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~250 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for SCN2A

Target: SCN2A (Sodium Channel, Voltage-Gated, Type II, alpha Subunit (SCN2A))

Alternative Name: Nav1.2 (SCN2A Products)

Synonyms: SCN2A2 antibody, 6430408L10 antibody, A230052E19Rik antibody, Nav1.2 antibody, Scn2a antibody, NachII antibody, RII/RIIA antibody, RNSCPIIR antibody, SCN antibody, SCN2A antibody, Scn2a2 antibody, ScpII antibody, BFIC3 antibody, BFIS3 antibody, BFNIS antibody, EIEE11 antibody, HBA antibody, HBSCI antibody, HBSCII antibody, NAC2 antibody, Na(v)1.2 antibody, SCN2A1 antibody, sodium voltage-gated channel alpha subunit 2 antibody, sodium channel protein type 2 subunit alpha antibody, sodium channel, voltage-gated, type II, alpha antibody, sodium channel, voltage-gated, type II, alpha subunit antibody, SCN2A antibody, LOC100217927 antibody, LOC100389294 antibody, LOC100484218 antibody, Scn2a antibody, LOC478773 antibody

Background:

Synonyms: SCN, SCN2A, ScpII, NachII, Nav1.2, Scn2a2, RII/RIIA, RNSCPIIR, Scn2a1, Sodium channel protein type 2 subunit alpha, Sodium channel protein brain II subunit alpha, Sodium channel protein type II subunit alpha, Voltage-gated Sodium channel subunit alpha Nav1.2

Background: Nav1.2 is a protein that in humans is encoded by the SCN2A gene. Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with 24 transmembrane domains and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. Neuronal Nav1.2 channels are therapeutic targets in seizure, pain and stroke.

Gene Name: Scn2a

Gene ID: 24766

NCBI Accession: NP_036779

UniProt: P04775