INA antibody

Catalog No. ABIN6658127

Supplier Product No.: 900-301-d04

This anti-INA antibody is a Mouse Monoclonal antibody detecting INA in WB, IHC, ELISA and FM. Suitable for Rat.

Quick Overview for INA antibody (ABIN6658127)

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Clone: 1D2

Product Details anti-INA Antibody

Supplier Product No.: 900-301-d04

Supplier: Rockland

Purpose: Alpha Internexin Antibody

Cross-Reactivity (Details): Anti-Alpha Internexin Antibody is directed against rat Alpha Internexin.

Purification: The antibody is protein G purified from cell culture supernatant.

Immunogen: Anti-Alpha Internexin Antibody was produced by repeated immunizations with recombinant rat alpha internexin expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:250

Western_Blot_Dilution: 1:2000

Comment:

Anti-Alpha Internexin antibody is tested for use in ELISA, Western Blotting, IHC and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 66kDa in size corresponding to alpha internexin proteins in the appropriate cell lysate or extract. Anti-Alpha Internexin antibody can be used on formalin-fixed cells in tissue culture, cryostat sections. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: Alpha Internexin

Background:

Synonyms: mouse anti-Alpha Internexin Antibody, Alpha INX, internexin, NF-66, NEF5

Background: Anti-Alpha Internexin antibody recognizes alpha-internexin which is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits. Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals. Anti-Alpha Internexin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: INA

Gene ID: 24503

UniProt: P23565