GLRA1 antibody (N-Term)

Catalog No. ABIN6658137

Supplier Product No.: 600-401-d65

This Rabbit Polyclonal antibody specifically detects GLRA1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for GLRA1 antibody (N-Term) (ABIN6658137)

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GLRA1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-d65

Supplier: Rockland

Purpose: Glycine Receptor Antibody

Cross-Reactivity (Details): Anti-Glycine Receptor Antibody is directed against rat Glycine Receptor protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Glycine Receptor Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Glycine Receptor (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 48 kDa in size corresponding to the alpha 1 and alpha 2 subunits of the glycine receptor in Western Blots of rat spinal cord and brain stem and in cell extracts.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 50μL

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for GLRA1

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Alternative Name: Glra1

Background:

Synonyms: Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit

Background: Glycine Receptor Antibody detects Glycine Receptor protein. Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor. Therefore, Glycine receptor antibody is ideal for investigators involved in neurological circuitry and more generally in Neuroscience.

Gene Name: GLRA1

Gene ID: 25674

NCBI Accession: NP_037265

UniProt: P07727

Pathways: Synaptic Membrane