CGRP antibody

Catalog No. ABIN6658144

Product Details anti-CGRP Antibody

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CGRP antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Rat (Rattus)

Purification: Anti-CGRP Monoclonal Antibody is directed against rat CGRP protein. The antibody was purified from concentrated tissue culture supernate by Protein G chromatography. This antibody is specific for human and rat CGRP protein. A BLAST analysis was used to suggest cross-reactivity with CGRP from most mammalian sources based on very high homology with the immunizing sequence. Calcitonin Gene Related Peptide antibody also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP).

Immunogen:

Immunogen: Anti-CGRP Monoclonal Antibody was produced by repeated immunizations with purified rat alpha-CGRP.

Immunogen Type: Recombinant Protein

Clone: 4901

Isotype: IgG2a

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50-1:100

Application Note: Anti-Calcitonin Gene Related Peptide antibody is suitable for use in ELISA and ICC. This product is not recommended for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Suggested positive controls are dorsal root ganglia, pancreas and gut.

Immunoprecipitation Dilution: 1:50-1:100

ELISA Dilution: 1:5,000

Western Blot Dilution: not recommended

IF Microscopy Dilution: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

References for anti-CGRP antibody (ABIN6658144)

Dhandapani, Arokiaraj, Taberner, Pacifico, Raja, Nocchi, Portulano, Franciosa, Maffei, Hussain, de Castro Reis, Reymond, Perlas, Garcovich, Barth, Johnsson, Lechner, Heppenstall: "Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons." in: Nature communications, Vol. 9, Issue 1, pp. 1640, (2019) (PubMed).

Target Details for CGRP

Target: CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))

Alternative Name: Calcitonin Gene Related Peptide (CGRP) (CALCA Products)

Background:

Synonyms: Alpha-type CGRP, Calcitonin gene-related peptide I

Background: CGRP antibody recognizes Calcitonin Gene Related Peptide or CGRP which is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known. CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches. CGRP antibodies have been used successfully in immunoneutralization. Anti-CGRP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CALCA

Gene ID: 24241

NCBI Accession: NP_001029128

UniProt: P01256

Pathways: Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour