CDK1 antibody (pTyr15)

Catalog No. ABIN6658147

Supplier Product No.: 600-401-160

The Rabbit Polyclonal anti-CDK1 antibody is suitable to detect CDK1 in samples from Rat. It has been validated for WB and ELISA.

Quick Overview for CDK1 antibody (pTyr15) (ABIN6658147)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDK1 Antibody

Binding Specificity: pTyr15

Supplier Product No.: 600-401-160

Supplier: Rockland

Purpose: cdc2 phospho Y15 Antibody

Cross-Reactivity (Details): Anti-cdc2 pY15 Antibody is directed against rat cdc2 protein phosphorylated at Y15.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-cdc2 pY15 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues surrounding Tyr 15.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-cdc2 pY15 antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 34 kDa in size corresponding to cdc2 protein in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: cdc2

Background:

Synonyms: CDC-like kinase 2, Clk2

Background: Cdc2 pY15 antibody recognizes Cdc2 which is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. Cdc2 expression in brain has been linked to both neurogenesis and apoptosis. Anti-Cdc2 pY15 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CLK2

Gene ID: 54237

NCBI Accession: NP_062169

UniProt: P39951

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA