FMR1 antibody (pSer499)

Catalog No. ABIN6658159

Supplier Product No.: 600-401-x07

The Rabbit Polyclonal anti-FMR1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect FMR1 in samples from Rat.

Quick Overview for FMR1 antibody (pSer499) (ABIN6658159)

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FMR1 Antibody

Binding Specificity: pSer499

Supplier Product No.: 600-401-x07

Supplier: Rockland

Purpose: FMRP Phospho S499 Antibody

Cross-Reactivity (Details): Anti-Anti-FMRP pS499 Antibody is directed against FMRP protein phosphorylated at S499.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser499 FMRP Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FRMP conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:250-1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser499 FMRP Antibody is tested for Western Blot and immunohistochemistry and is specific for the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: FMRP

Background:

Synonyms: Fragile X mental retardation protein 1 homolog, FMR-1

Background: Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits. Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation. FMRP pS499 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: FMR1

Gene ID: 24948

NCBI Accession: NP_434691

UniProt: Q80WE1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development