Neuroligin 1 antibody (AA 718-843) (FITC)

Catalog No. ABIN6658176

Supplier Product No.: 200-302-w75

This Mouse Monoclonal antibody specifically detects Neuroligin 1 in WB, IHC and ELISA. It exhibits reactivity toward Rat.

Quick Overview for Neuroligin 1 antibody (AA 718-843) (FITC) (ABIN6658176)

Target: Neuroligin 1 (NLGN1)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neuroligin 1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: S97A-31

Product Details anti-Neuroligin 1 Antibody (FITC)

Binding Specificity: AA 718-843

Supplier Product No.: 200-302-w75

Supplier: Rockland

Purpose: Neuroligin 1 Antibody Fluorescein

Cross-Reactivity (Details): BLAST analysis suggests that it is 99 % identical to mouse, 99 % identical to human and >50 % identical to Neuroligin-2 and -3.

Purification: Anti-Neuroligin 1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Neuroligin 1 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 718-843 of rat Neuroligin-1.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:5,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:500

Comment:

Anti-Neuroligin 1 FITC Conjugated Antibody is suitable for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~120kDa on specific lysates or tissues. Does not cross react with other Neuroligins. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Alternative Name: Neuroligin 1

Background:

Synonyms: NLG 1, KIAA1070, MGC45115, Neuroligin-1, NL1, NLG1, Nlgn1

Background: Neuroligin-1 is a neuronal cell surface protein belonging to the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses for their normal, functional development, and is necessary to the regulation of synaptic plasticity and the development of long-term memory within the adult amygdala in mammals. It is believed to participate in cell-cell-interaction through the assembly of intracellular junction by the binding of beta-neurexins, and may also be a factor in the maintenance and assembly of synaptic junctions. It is also thought to have involvement in excitatory synaptic specification. Within brain tissue, Neuroligin-1 is primarily observed in neurons and spinal cord. Anti-Neuroligin-1 is ideal for research in Neuroscience, including narcolepsy and synaptic neurotransmission: GABAergic inhibition, as well as Cell Adhesion.

Gene Name: Neuroligin-1

Gene ID: 116647

UniProt: Q62765

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis