GABRD antibody (Internal Region)

Catalog No. ABIN6658204

Supplier Product No.: 200-301-f33

The Mouse Monoclonal anti-GABRD antibody has been validated for WB, IHC, ELISA, IF and FM. It is suitable to detect GABRD in samples from Rat.

Quick Overview for GABRD antibody (Internal Region) (ABIN6658204)

Target: GABRD (GABA A Receptor delta (GABRD))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABRD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: N151-3

Product Details anti-GABRD Antibody

Binding Specificity: Internal Region

Supplier Product No.: 200-301-f33

Supplier: Rockland

Purpose: GABA-A Receptor delta Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with GABA-A receptor delta from mouse and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-GABA-A receptor delta Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: GABA-A receptor-delta Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to an internal region of rat GABA-A-R-Delta.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:5000-1:25,000

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Comment:

Anti-GABA-A receptor delta Antibody is tested for use in WB and IF microscopy. Expect a band approximately ~55kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GABRD

Target: GABRD (GABA A Receptor delta (GABRD))

Alternative Name: GABA-A receptor-delta

Background:

Synonyms: GABAA-RD, Gabrd, MGC105467, Gamma-aminobutyric acid receptor subunit delta, GABA(A) receptor subunit delta

Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain.

Gene Name: Gabrd

Gene ID: 29689

NCBI Accession: NP_058985

UniProt: P18506