SNAP25 antibody (pSer187)

Catalog No. ABIN6658206

Supplier Product No.: 600-401-x28

This Rabbit Polyclonal antibody specifically detects SNAP25 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for SNAP25 antibody (pSer187) (ABIN6658206)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SNAP25 Antibody

Binding Specificity: pSer187

Supplier Product No.: 600-401-x28

Supplier: Rockland

Purpose: SNAP25 Phospho S187 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: Anti-Phospho-Ser187 SNAP25 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser187 SNAP25 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 of rat SNAP25 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser187 SNAP25 Antibody is tested for Western Blots and is specific for the ~25 kDa SNAP25 protein phosphorylated at Ser187 in Western blots. Immunolabeling is completely blocked by blocked lambda-Phosphatase. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background:

Synonyms: Synaptosomal-associated protein 25, SNAP-25, Super Protein, SUP, Synaptosoma-associated 25 kDa protein

Background: SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer's Disease. In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia. Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs). Activation of PKC results in the phosphorylation of SNAP-25 on ser187. Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC. Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability. SNAP25 pS187 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: SNAP25

Gene ID: 25012

NCBI Accession: NP_001257504

UniProt: P60881

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport