STXBP2 antibody (pSer515)

Catalog No. ABIN6658248

Supplier Product No.: 600-401-x16

Product Details anti-STXBP2 Antibody

Target: STXBP2 (Syntaxin Binding Protein 2 (STXBP2))

Binding Specificity: pSer515

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-x16

Supplier: Rockland

Purpose: Munc18 Phospho S515 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser515 Munc18 Antibody is directed against Munc18 protein phosphorylated at S515.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser515 Munc18 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser515 of rat Munc18 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser515 Munc18 Antibody is tested for Western Blot, and it is specific for the ~65 kDa Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for STXBP2

Target: STXBP2 (Syntaxin Binding Protein 2 (STXBP2))

Alternative Name: Stxbp2 (STXBP2 Products)

Background:

Synonyms: Syntaxin-binding protein 2, Protein unc-18 homolog 2, Unc18-2, Protein unc-18 homolog B, Unc-18B

Background: Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin. Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex. The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein. Munc-18 pS515 Antibody is ideal for researchers interested in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: STXBP2

Gene ID: 81804

NCBI Accession: NP_112388

UniProt: Q62753

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis