RPH3A antibody (C-Term)

Catalog No. ABIN6658251

Product Details anti-RPH3A Antibody

Target: RPH3A (Rabphilin 3A (RPH3A))

Binding Specificity: C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPH3A antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity with Rab 3A from other species has not been determined, however, the sequence of the immunogen shows 100 % identity to mouse, human, bovine, canine, and non-human primates, therefore, cross reactivity is expected.

Purification: Anti-Rabphilin 3A antibody is directed against rat rabphilin 3A proteins. Anti-Rabphilin 3A antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen:

Immunogen: Anti-Rabphilin 3A Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Rabphilin 3A (Rabbit) antibody is suitable for use in ELISA, and in western blotting. Expect a band of approximately 82 kDa in size corresponding to rabphilin 3A protein in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Target Details for RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: Rabphilin 3A (RPH3A Products)

Synonyms: 2900002P20Rik antibody, AU022689 antibody, AW108370 antibody, CG11556 antibody, Dmel\\CG11556 antibody, exophilin-1 antibody, fc61h10 antibody, rph3a antibody, wu:fc61h10 antibody, rabphilin 3A antibody, Rabphilin antibody, rabphilin-3a, putative antibody, rabphilin-3A antibody, rabphilin 3A S homeolog antibody, rabphilin 3A homolog (mouse), a antibody, RPH3A antibody, Rph3a antibody, Rph antibody, Smp_145120 antibody, Tsp_00335 antibody, rph3a.S antibody, rph3aa antibody

Background:

Synonyms: Rabphilin 3A Homolog, Rabphilin-3A, RP3A, Rph3A, Exophilin 1, Exophilin-1

Background: Rabphilin 3A antibody detects Rab 3A which is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes. The expression of rabphilin 3A is reduced in an animal model of Huntington's disease. Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner. Anti-Rabphilin 3A antibody is ideal for investigators involved in Signal Transduction, Cell Signaling, and Neuroscience research.

Gene Name: RPH3A

Gene ID: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis