MAP2K5 antibody (pSer311, pThr315)

Catalog No. ABIN6658265

Supplier Product No.: 612-401-d74

This anti-MAP2K5 antibody is a Rabbit Polyclonal antibody detecting MAP2K5 in WB. Suitable for Rat.

Quick Overview for MAP2K5 antibody (pSer311, pThr315) (ABIN6658265)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAP2K5 Antibody

Binding Specificity: pSer311, pThr315

Supplier Product No.: 612-401-d74

Supplier: Rockland

Purpose: MEK5 phospho S311/phospho T315 Antibody

Cross-Reactivity (Details): MEK5 antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Purification: Anti-MEK5 pS311/pT315 antibody is directed against rat MEK5.

Immunogen: Anti-MEK5 pS311/pT315 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-MEK5 pS311/pT315 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 49 kDa in size corresponding to MEK5 protein phosphorylated at Ser311/Thr315 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MEK5

Background:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5

Background: MEK5 pS311/pT315 antibody detects MEK5 that is phosphorylated at Ser311 and Threonine315. MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 whereas MEK5 itself is regulated by MEKK3. An important link between MEK5 and metastatic prostrate cancer has been demonstrated. Dual phosphorylation of Ser311 and Thr315 have been implicated in cell proliferation. Therefore, MEK5 pS311/pT315 antibody is ideal for investigators involved in Cell Signaling, Cell Migration and Proliferation and, more generally in Cancer Research.

Gene Name: MAP2K5

Gene ID: 29568

UniProt: Q62862

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway