PAK1/2/3 antibody (pThr402)

Catalog No. ABIN6658268

Product Details anti-PAK1/2/3 Antibody

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Binding Specificity: pThr402

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity with PAK from other species has not been determined.

Purification: Anti-PAK 1/2/3 pT402 antibody is directed against rat PAK protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification and is specific for the phosphorylated protein. The immunolabeling of PAK is completely eliminated by lambda-phosphatase treatment. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, human and mouse.

Immunogen:

Immunogen: Anti-PAK 1/2/3 pT402 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-PAK 1/2/3 pT402 Antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 68-70 kDa in size corresponding to PAK proteins phosphorylated at Thr402 in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK 1/2/3 (PAK1/2/3 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, pak1.S antibody, PAK1 antibody

Background:

Synonyms: Serine/threonine-protein kinase PAK 1, Alpha-PAK, Protein kinase MUK2, p21-activated kinase 1, p68-PAK

Background: PAK 1/2/3 pT402 Antibody detects PAK 1/2/3 pT402 proteins. In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously. Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death. Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK. Anti-PAK 1/2/3 pT402 Antibody is ideal for investigators involved in Neuroscience and Apoptosis research.

Gene Name: PAK1-3

Gene ID: 29431

UniProt: P35465