LAMP1 antibody

Catalog No. ABIN6658286

Supplier Product No.: 200-301-g09

This anti-LAMP1 antibody is a Mouse Monoclonal antibody detecting LAMP1 in WB, IHC, IF and IP. Suitable for Rat.

Quick Overview for LAMP1 antibody (ABIN6658286)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: Ly1C6

Product Details anti-LAMP1 Antibody

Supplier Product No.: 200-301-g09

Supplier: Rockland

Purpose: LAMP1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with LAMP1 from Hamster and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-LAMP1 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: LAMP1 Antibody was produced in mice by repeated immunizations raised against rat liver lysosomal membrane preparations.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-LAMP1 Antibody is tested for WB, IP, and IF microscopy. Expect a band approximately ~120kDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: LAMP1

Background:

Synonyms: CD107, CD107a, LAMPA, LGP120, IgpA, Lysosome-associated membrane glycoprotein 1, CD107 antigen-like family member A, 120 kDa lysosomal membrane glycoprotein, Lamp-1

Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation. Ideal for investigators involved in cytokines, growth factors and cancer, as well as, Chaperones and Organelle Markers research.

Gene Name: LAMP1

Gene ID: 25328

NCBI Accession: NP_036989

UniProt: P14562

Pathways: Autophagy