TIMP3 antibody (AA 151-211)

Catalog No. ABIN668361

Cited in 5+ publications. The Rabbit Polyclonal anti-TIMP3 antibody (ABIN668361) specifically detects TIMP3 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). The antibody is reactive with Human, Rat and Rabbit samples.

Quick Overview for TIMP3 antibody (AA 151-211) (ABIN668361)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TIMP3 Antibody

Binding Specificity: AA 151-211

Cross-Reactivity: Human, Rabbit, Rat

Predicted Reactivity: Mouse,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TIMP-3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TIMP3 antibody (ABIN668361)

Han, Liang, Yi, Tan, He, Wang, Li, Wu, Ma, Shi, Guo, Bai: "Long-Term Selenium-Deficient Diet Induces Liver Damage by Altering Hepatocyte Ultrastructure and MMP1/3 and TIMP1/3 Expression in Growing Rats." in: Biological trace element research, (2016) (PubMed).

Peng, Xin, Han, Gao, Gao, Lei, Ji, An, Cao: "Expression and regulative function of tissue inhibitor of metalloproteinase 3 in the goat ovary and its role in cultured granulosa cells." in: Molecular and cellular endocrinology, Vol. 412, pp. 104-15, (2015) (PubMed).

Peng, Gao, Gao, Lei, Han, Xin, An, Cao: "Expression and regulation of tissue inhibitors of metalloproteinases (TIMP1 and TIMP3) in goat oviduct." in: Theriogenology, Vol. 84, Issue 9, pp. 1636-43, (2015) (PubMed).

Lu, Cao, Liu, Li, Chen, Fu, Zhang, Liu, Luo, Wang, Li, Caterson: "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." in: Cells, tissues, organs, Vol. 196, Issue 3, pp. 241-50, (2012) (PubMed).

Liu, Cui, Ao, Zhou, Zhou, Yuan, Xiang, Liu, Cao et al.: "Aberrant methylation accounts for cell adhesion-related gene silencing during 3-methylcholanthrene and diethylnitrosamine induced multistep rat lung carcinogenesis associated with overexpression of ..." in: Toxicology and applied pharmacology, Vol. 251, Issue 1, pp. 70-8, (2011) (PubMed).

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP-3

Background:

Synonyms: SFD, K222, K222TA2, HSMRK222, Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

Background: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Gene ID: 7078

UniProt: P35625