Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Donkey anti-Human IgG Antibody (Cy2) - Preadsorbed

ELISA, WB, FACS, FLISA, FM Polyclonal IgG Cy2
Rockland
Catalog No. ABIN6698908
Supplier Product No.: 609-711-123

Quick Overview for Donkey anti-Human IgG Antibody (Cy2) - Preadsorbed (ABIN6698908)

Target

IgG

Clonality

  • 3437
  • 230
  • 16
  • 1
Polyclonal

Conjugate

  • 665
  • 422
  • 422
  • 398
  • 274
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 39
  • 39
  • 37
  • 35
  • 35
  • 32
  • 31
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
Cy2

Application

  • 2118
  • 2050
  • 1884
  • 1872
  • 999
  • 919
  • 895
  • 520
  • 424
  • 234
  • 234
  • 149
  • 102
  • 98
  • 88
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotype

IgG
  • Reactivity

    • 752
    • 703
    • 607
    • 468
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 82
    • 62
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 1773
    • 1583
    • 566
    • 282
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 16
    • 11
    • 11
    Donkey

    Supplier Product No.

    609-711-123

    Supplier

    Rockland

    Purpose

    Human IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed

    Cross-Reactivity (Details)

    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins

    Characteristics

    Donkey Anti Human IgG CY2 Conjugated Antibody, Donkey Anti-Human IgG Antibody CY2 Antibody,Anti-Human IgG (H&L) Cy2 generated in donkey detects human Immunoglobulin G (heavy and light chains).

    Purification

    Preadsorption: Pre-Adsorbed

    Immunogen

    Human IgG whole molecule
  • Application Notes

    FLISA_Dilution: 1:10,000 - 1:50,000

    ELISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Western_Blot_Dilution: 1:1,000 - 1:5,000

    Comment

    Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    1.0 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Storage

    4 °C,-20 °C

    Storage Comment

    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Expiry Date

    12 months
  • Target

    IgG

    Target Type

    Antibody

    Background

    It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
You are here:
Chat with us!