Goat anti-Monkey IgG (Chain gamma) Antibody (DyLight 488) - Preadsorbed

Catalog No. ABIN6698964

Supplier Product No.: 617-141-012

Quick Overview for Goat anti-Monkey IgG (Chain gamma) Antibody (DyLight 488) - Preadsorbed (ABIN6698964)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 488

Application: Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Chain gamma

Reactivity: Monkey

Host: Goat

Supplier Product No.: 617-141-012

Supplier: Rockland

Characteristics:

Synonyms: goat anti-Monkey IgG (gamma chain) DyLight™488 conjugated antibody, goat anti-Monkey IgG gamma antibody DyLight™ 488 conjugation

Background: Anti-Monkey IgG (gamma chain) DyLight™488 conjugated antibody generated in goat detects specifically monkey IgG (gamma chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. This DyLight™488 conjugated anti-Monkey IgG gamma chain secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Monkey IgG (gamma chain) was produced by repeated immunization with monkey IgG gamma heavy chain in goat.

Immunogen Type: Native Protein

Labeling Ratio: 5.7

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Monkey IgG (gamma chain) DyLight™488 is suitable for use in immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity and signal detection should be optimized by the end user.

FLISA Dilution: >1:20,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody