Goat anti-Mouse IgG Antibody (Cy5.5) - Preadsorbed

Catalog No. ABIN6698988

Supplier Product No.: 610-113-121

Quick Overview for Goat anti-Mouse IgG Antibody (Cy5.5) - Preadsorbed (ABIN6698988)

Target: IgG

Clonality: Polyclonal

Conjugate: Cy5.5

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-113-121

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Characteristics: Goat Anti-Mouse IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed, Goat Anti Mouse IgG Antibody CY5.5 Conjugated,Anti-Mouse IgG Cy5.5 Antibody generated in goat detects reactivity to Mouse IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 4.3

Application Details

Application Notes:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Comment:

Anti-Mouse IgG Cy5.5 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Gawronska-Kozak, Kopcewicz, Machcinska-Zielinska, Walendzik, Wisniewska, Drukała, Wasniewski, Rutkowska, Malinowski, Pulinski: "Gender Differences in Post-Operative Human Skin." in: Biomedicines, Vol. 11, Issue 10, (2023) (PubMed).

Machcinska, Walendzik, Kopcewicz, Wisniewska, Rokka, Pääkkönen, Slowinska, Gawronska-Kozak: "Hypoxia reveals a new function of Foxn1 in the keratinocyte antioxidant defense system." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 36, Issue 8, pp. e22436, (2022) (PubMed).

Machcinska, Kopcewicz, Bukowska, Walendzik, Gawronska-Kozak: "Impairment of the Hif-1α regulatory pathway in Foxn1-deficient (Foxn1-/- ) mice affects the skin wound healing process." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 35, Issue 2, pp. e21289, (2021) (PubMed).

Kur-Piotrowska, Bukowska, Kopcewicz, Dietrich, Nynca, Slowinska, Gawronska-Kozak: "Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing." in: Scientific reports, Vol. 8, Issue 1, pp. 5425, (2018) (PubMed).

Yamashita, Ancillotti, Rangel, Fontenele, Figueiredo-Freitas, Possidonio, Soares, Sorenson, Mermelstein, Nogueira: "Balance between S-nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation." in: American journal of physiology. Cell physiology, Vol. 313, Issue 1, pp. C11-C26, (2017) (PubMed).

Markoullis, Bulian, Hölzlwimmer, Quintanilla-Martinez, Heiliger, Zitzelsberger, Scherb, Mysliwietz, Uphoff, Drexler, Adler, Busch, Schmidt, Mahabir: "Mycoplasma contamination of murine embryonic stem cells affects cell parameters, germline transmission and chimeric progeny." in: Transgenic research, Vol. 18, Issue 1, pp. 71-87, (2009) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.