Goat anti-Rat IgG Antibody (DyLight 649)

Catalog No. ABIN6699155

Supplier Product No.: 612-143-002

Quick Overview for Goat anti-Rat IgG Antibody (DyLight 649) (ABIN6699155)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Supplier Product No.: 612-143-002

Supplier: Rockland

Purpose: Rat IgG (H&L) Antibody Dylight™ 649 Conjugated

Characteristics: Goat Anti-Rat IgG DyLight 649™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 649™ Conjugation,

Immunogen: Rat IgG, whole molecule

Labeling Ratio: 2.9

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Rat IgG DyLight™ 649 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Riedemann, Sutor, Bergami, Riedemann: "Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line." in: The Journal of comparative neurology, Vol. 527, Issue 14, pp. 2215-2232, (2020) (PubMed).

Riedemann, Straub, Sutor: "Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex." in: PLoS ONE, Vol. 13, Issue 7, pp. e0200567, (2019) (PubMed).

Riedemann, Schmitz, Sutor: "Immunocytochemical heterogeneity of somatostatin-expressing GABAergic interneurons in layers II and III of the mouse cingulate cortex: A combined immunofluorescence/design-based stereologic study." in: The Journal of comparative neurology, Vol. 524, Issue 11, pp. 2281-99, (2017) (PubMed).

Target Details

Target: IgG

Target Type: Antibody