Goat anti-Rat IgG Antibody (DyLight 800)

Catalog No. ABIN6699167

Supplier Product No.: 612-145-002

Quick Overview for Goat anti-Rat IgG Antibody (DyLight 800) (ABIN6699167)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Supplier Product No.: 612-145-002

Supplier: Rockland

Purpose: Rat IgG (H&L) Antibody Dylight™ 800 Conjugated

Characteristics: Goat Anti-Rat IgG DyLight 800™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 800™ Conjugation,

Purification:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. This antibody will react with heavy chains of Rat IgG and with light chains of most Rat immunoglobulins.

Immunogen: Rat IgG, whole molecule

Labeling Ratio: 2.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Rat IgG DyLight™ 800 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Voß, Klaus, Lichti, Ganter, Guizetti: "Malaria parasite centrins can assemble by Ca2+-inducible condensation." in: PLoS pathogens, Vol. 19, Issue 12, pp. e1011899, (2024) (PubMed).

Szewczyk, Günther, Japtok, Frech, Naumann, Lee, Hermann: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).

Balic, Albargy, Luu, Kirby, Jayasekara, Mansell, Garama, De Nardo, Baschuk, Louis, Humphries, Fitzgerald, Latz, Gough, Mansell: "STAT3 serine phosphorylation is required for TLR4 metabolic reprogramming and IL-1β expression." in: Nature communications, Vol. 11, Issue 1, pp. 3816, (2020) (PubMed).

Bacman, Kauppila, Pereira, Nissanka, Miranda, Pinto, Williams, Larsson, Stewart, Moraes: "MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation." in: Nature medicine, Vol. 24, Issue 11, pp. 1696-1700, (2019) (PubMed).

Bacman, Kauppila, Pereira, Nissanka, Miranda, Pinto, Williams, Larsson, Stewart, Moraes: "Author Correction: MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation." in: Nature medicine, Vol. 24, Issue 12, pp. 1940, (2018) (PubMed).

Target Details

Target: IgG

Target Type: Antibody