Cyclin B1 antibody (AA 271-433) (Cy3)

Catalog No. ABIN670299

This anti-Cyclin B1 antibody is a Rabbit Polyclonal antibody detecting Cyclin B1 in WB, FACS, IF (cc) and IF (p). Suitable for Human, Mouse, Rat, Cow and Chicken.

Quick Overview for Cyclin B1 antibody (AA 271-433) (Cy3) (ABIN670299)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Cyclin B1 Antibody (Cy3)

Binding Specificity: AA 271-433

Cross-Reactivity: Chicken, Cow, Human, Mouse, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CCNB1

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background:

Synonyms: CycB1, Cycb-4, Cycb-5, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13, G2/mitotic-specific cyclin-B1, Ccnb1, Ccn-2, Cycb

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Gene ID: 268697

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase