Connexin 43/GJA1 antibody (AA 211-260)

Catalog No. ABIN671451

This anti-Connexin 43/GJA1 antibody is a Rabbit Polyclonal antibody detecting Connexin 43/GJA1 in WB, ELISA, IHC (p), IF (p), FACS, IF (cc) and IHC (fro). Suitable for Human, Rat, Mouse and Dog. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Connexin 43/GJA1 antibody (AA 211-260) (ABIN671451)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: AA 211-260

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Connexin-43

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Connexin 43/GJA1 antibody (ABIN671451)

Zhao, Xu, Yun, Zhao, Li, Gong, Yuan, Yan, Zhang, Ding, Wang, Zhang, Dong, Xiu, Yang, Liu, Xue, Li: "Chronic obstructive sleep apnea causes atrial remodeling in canines: mechanisms and implications." in: Basic research in cardiology, Vol. 109, Issue 5, pp. 427, (2014) (PubMed).

Chen, Gao, Ni, Dai: "Carbenoxolone pretreatment and treatment of posttraumatic epilepsy." in: Neural regeneration research, Vol. 8, Issue 2, pp. 169-76, (2014) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43

Background:

Synonyms: HSS, CMDR, CX43, GJAL, ODDD, AVSD3, HLHS1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, GJA1

Background: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity).

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization