FGFR4 antibody (AA 1-100) (Cy5.5)

Catalog No. ABIN671771

The Rabbit Polyclonal anti-FGFR4 antibody has been validated for IF (p) and IF (cc). It is suitable to detect FGFR4 in samples from Human and Rat.

Quick Overview for FGFR4 antibody (AA 1-100) (Cy5.5) (ABIN671771)

Target: FGFR4 (Fibroblast Growth Factor Receptor 4 (FGFR4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGFR4 antibody is conjugated to Cy5.5

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-FGFR4 Antibody (Cy5.5)

Binding Specificity: AA 1-100

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FGFR4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FGFR4

Target: FGFR4 (Fibroblast Growth Factor Receptor 4 (FGFR4))

Alternative Name: FGFR4/CD334

Background:

Synonyms: TKF, JTK2, CD334, Fibroblast growth factor receptor 4, FGFR-4, FGFR4

Background: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation, MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

Gene ID: 2264

UniProt: P22455

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding