SYVN1 antibody (AA 531-617)

Catalog No. ABIN671811

This anti-SYVN1 antibody is a Rabbit Polyclonal antibody detecting SYVN1 in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human and Mouse. This Primary Antibody has been cited in 3+ publications.

Quick Overview for SYVN1 antibody (AA 531-617) (ABIN671811)

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYVN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SYVN1 Antibody

Binding Specificity: AA 531-617

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SYVN1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SYVN1 antibody (ABIN671811)

Yang, He, Ma, Zhang, Zhu, Wan, Wang, Wang, Liu, Li: "Experimental study of the protective effects of SYVN1 against diabetic retinopathy." in: Scientific reports, Vol. 5, pp. 14036, (2016) (PubMed).

Zemoura, Schenkel, Acuña, Yévenes, Zeilhofer, Benke: "Endoplasmic reticulum-associated degradation controls cell surface expression of ?-aminobutyric acid, type B receptors." in: The Journal of biological chemistry, Vol. 288, Issue 48, pp. 34897-905, (2013) (PubMed).

Yan, Zheng, Chen, Liu, Li, Hu, Pei, Li: "Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats." in: Experimental diabetes research, Vol. 2012, pp. 743780, (2011) (PubMed).

Target Details for SYVN1

Target: SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

Alternative Name: SYVN1

Background:

Synonyms: DER3, HRD1, E3 ubiquitin-protein ligase synoviolin, Synovial apoptosis inhibitor 1, SYVN1, KIAA181

Background: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.

Gene ID: 84447

UniProt: Q86TM6

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling