CCDC90A antibody

Catalog No. ABIN6719261

The Rabbit Polyclonal anti-CCDC90A antibody has been validated for WB and IHC. It is suitable to detect CCDC90A in samples from Human, Mouse and Rat.

Quick Overview for CCDC90A antibody (ABIN6719261)

Target: CCDC90A (MCUR1) (Mitochondrial Calcium Uniporter Regulator 1 (MCUR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCDC90A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CCDC90A Antibody

Purpose: Anti-MCUR1 Antibody Picoband®

Sequence: ATQQAEIIVS ALVKILEANM DIVYKDMVTK MQQE

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MCUR1 Antibody Picoband® (ABIN6719261). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MCUR1, which shares 79.4% and 82.3% amino acid (aa) sequence identity with mouse and rat MCUR1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
1. Chaudhuri, D., Artiga, D. J., Abiria, S. A., Clapham, D. E. Mitochondrial calcium uniporter regulator 1 (MCUR1) regulates the calcium threshold for the mitochondrial permeability transition. Proc. Nat. Acad. Sci. 113: E1872-E1880, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 5/23/2016. 3. Mallilankaraman, K., Cardenas, C., Doonan, P. J., Chandramoorthy, H. C., Irrinki, K. M., Golenar, T., Csordas, G., Madireddi, P., Yang, J., Muller, M., Miller, R., Kolesar, J. E., Molgo, J., Kaufman, B., Hajnoczky, G., Foskett, J. K., Madesh, M. MCUR1 is an essential component of mitochondrial Ca(2+) uptake that regulates cellular metabolism. Nature Cell Biol. 14: 1336-1343, 2012. Note: Erratum: Nature Cell Biol. 15: 123 only, 2013. Erratum: Nature Cell Biol. 17: 953 only, 2015.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCDC90A

Target: CCDC90A (MCUR1) (Mitochondrial Calcium Uniporter Regulator 1 (MCUR1))

Alternative Name: MCUR1

Background:

Synonyms: Mitochondrial calcium uniporter regulator 1, MCU regulator 1, Coiled-coil domain-containing protein 90A, mitochondrial, MCUR1, C6orf79, CCDC90A

Tissue Specificity: Ubiquitously expressed.

Background: MCUR1 is an inner mitochondrial membrane protein that has been shown to function as a component of the mitochondrial Ca (2+) uniporter, in the assembly of mitochondrial respiratory complex IV, and in mitochondrial permeability transition (MPT). The MCUR1 gene is mapped to chromosome 6p23 based on an alignment of the MCUR1 sequence with the genomic sequence.

Molecular Weight: 40 kDa

Gene ID: 63933