DARS2 antibody (AA 334-448)
-
- Target See all DARS2 Antibodies
- DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))
-
Binding Specificity
- AA 334-448
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This DARS2 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for DARS2 detection. Tested with WB, IHC-P, Direct ELISA in Human.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Rabbit IgG polyclonal antibody for DARS2 detection. Tested with WB, IHC-P, Direct ELISA in Human.
- Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human DARS2 recombinant protein (Position: D334-A448).
- Isotype
- IgG
- Top Product
- Discover our top product DARS2 Primary Antibody
-
-
- Application Notes
-
Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL
- Comment
-
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- Target
- DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))
- Alternative Name
- DARS2 (DARS2 Products)
- Synonyms
- si:dkey-21n10.1 antibody, 5830468K18Rik antibody, RGD1308286 antibody, ASPRS antibody, LBSL antibody, MT-ASPRS antibody, RP3-383J4.2 antibody, aspartyl-tRNA synthetase 2, mitochondrial antibody, aspartate--tRNA ligase, mitochondrial antibody, aspartyl-tRNA synthetase 2 (mitochondrial) antibody, DARS2 antibody, dars2 antibody, LOC100547893 antibody, Dars2 antibody
- Background
-
Synonyms: Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, DARS2
Background: DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.
- Gene ID
- 55157
-