DARS2 antibody (AA 334-448)

Catalog No. ABIN6719279

Product Details anti-DARS2 Antibody

Target: DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))

Binding Specificity: AA 334-448

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for DARS2 detection. Tested with WB, IHC-P, Direct ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for DARS2 detection. Tested with WB, IHC-P, Direct ELISA in Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DARS2 recombinant protein (Position: D334-A448).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DARS2

Target: DARS2 (Aspartyl-tRNA Synthetase 2, Mitochondrial (DARS2))

Alternative Name: DARS2 (DARS2 Products)

Synonyms: si:dkey-21n10.1 antibody, 5830468K18Rik antibody, RGD1308286 antibody, ASPRS antibody, LBSL antibody, MT-ASPRS antibody, RP3-383J4.2 antibody, aspartyl-tRNA synthetase 2, mitochondrial antibody, aspartate--tRNA ligase, mitochondrial antibody, aspartyl-tRNA synthetase 2 (mitochondrial) antibody, DARS2 antibody, dars2 antibody, LOC100547893 antibody, Dars2 antibody

Background:

Synonyms: Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, DARS2

Background: DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). The International Radiation Hybrid Mapping Consortium mapped the DARS2 gene to chromosome 1.

Gene ID: 55157