Cardiac Troponin T2 antibody (AA 88-298)

Catalog No. ABIN6719304

This anti-Cardiac Troponin T2 antibody is a Rabbit Polyclonal antibody detecting Cardiac Troponin T2 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for Cardiac Troponin T2 antibody (AA 88-298) (ABIN6719304)

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cardiac Troponin T2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cardiac Troponin T2 Antibody

Binding Specificity: AA 88-298

Purpose: Anti-Cardiac Troponin T/TNNT2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cardiac Troponin T/TNNT2 Antibody Picoband® (ABIN6719304). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Cardiac Troponin T recombinant protein (Position: K88-K298).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Aviles, R. J., Askari, A. T., Lindahl, B., Wallentin, L., Jia, G., Ohman, E. M., Mahaffey, K. W., Newby, L. K., Califf, R. M., Simoons, M. L., Topol, E. J., Lauer, M. S. Troponin T levels in patients with acute coronary syndromes, with or without renal dysfunction. New Eng. J. Med. 346: 2047-2052, 2002. 2. Charlet-B, N., Logan, P., Singh, G., Cooper, T. A. Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Molec. Cell 9: 649-658, 2002. 3. Du, C.-K., Morimoto, S., Nishii, K., Minakami, R., Ohta, M., Tadano, N., Lu, Q.-W., Wang, Y.-Y., Zhan, D.-Y., Mochizuki, M., Kita, S., Miwa, Y., Takahashi-Yanaga, F., Iwamoto, T., Ohtsuki, I., Sasaguri, T. Knock-in mouse model of dilated cardiomyopathy caused by troponin mutation.Circ. Res. 101: 185-194, 2007.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Cardiac Troponin T2

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Alternative Name: TNNT2

Background:

Synonyms: Troponin T, cardiac muscle, TnTc, Cardiac muscle troponin T, cTnT, TNNT2

Tissue Specificity: Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

Background: Cardiac muscle troponin T (cTnT), is a protein which in humans is encoded by the TNNT2 gene. It is mapped to 1q32.1. The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.

Molecular Weight: 43 kDa

Gene ID: 7139

UniProt: P45379