NFIA antibody (Middle Region)

Catalog No. ABIN6719349

The Mouse Monoclonal anti-NFIA antibody (Clone 16H11) (ABIN6719349) specifically detects NFIA in WB, IHC, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for NFIA antibody (Middle Region) (ABIN6719349)

Target: NFIA (Nuclear Factor I/A (NFIA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 16H11

Product Details anti-NFIA Antibody

Binding Specificity: Middle Region

Purpose: Anti-NFIA Antibody (monoclonal, 16H11)

Sequence: AYFVHAADSS QSESPSQPSD ADIKDQPENG HLGFQDSFVT SGVFS

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by one amino acid,and identical to the related rat sequence.

Characteristics: Anti-NFIA Antibody.(monoclonal, 16H11). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NFIA, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL Immunocytochemistry/Immunofluorescence, 2 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NFIA

Target: NFIA (Nuclear Factor I/A (NFIA))

Alternative Name: NFIA

Background:

Background: Nuclear factor 1 A-type?is a?protein?that in?humans?is encoded?by the?NFIA?gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins?with similar, and possibly identical, DNA-binding specificity. They function as cellular?transcription factors?and as replication factors for?adenovirus?DNA replication. Diversity in this protein family is generated by multiple?genes, differential?splicing, and heterodimerization.

Gene Full Name: nuclear factor I A

Molecular Weight: 62 kDa

Gene ID: 4774

UniProt: Q12857