RAB11B antibody

Catalog No. ABIN6719364

This anti-RAB11B antibody is a Rabbit Polyclonal antibody detecting RAB11B in WB, IHC, ICC, FACS and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for RAB11B antibody (ABIN6719364)

Target: RAB11B (RAB11B, Member RAS Oncogene Family (RAB11B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB11B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RAB11B Antibody

Purpose: Anti-RAB11B Antibody Picoband®

Sequence: QKQIADRAAH DESPGNNVVD ISVPPTTDGQ KPNKLQCC

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAB11B Antibody Picoband® (ABIN6719364). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB11B, which shares 97.4% and 100% amino acid (aa) sequence identity with mouse and rat RAB11B, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2014. 2. Lamers, I. J. C., Reijnders, M. R. F., Venselaar, H., Kraus, A., DDD Study, Jansen, S., de Vries, B. B. A., Houge, G., Gradek, G. A., Seo, J., Choi, M., Chae, J.-H., and 11 others. Recurrent de novo mutations disturbing the GTP/GDP binding pocket of RAB11B cause intellectual disability and a distinctive brain phenotype. Am. J. Hum. Genet. 101: 824-832, 2017. 3. Zhu, A. X., Zhao, Y., Flier, J. S. Molecular cloning of two small GTP-binding proteins from human skeletal muscle. Biochem. Biophys. Res. Commun. 205: 1875-1882, 1994.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAB11B

Target: RAB11B (RAB11B, Member RAS Oncogene Family (RAB11B))

Alternative Name: RAB11B

Background:

Synonyms: Ras-related protein Rab-11B, GTP-binding protein YPT3, RAB11B, YPT3

Tissue Specificity: Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Background: Ras-related protein Rab-11B is a protein that in humans is encoded by the RAB11B gene. It is mapped to 19p13.2. The Ras superfamily of small GTP-binding proteins, which includes the Ras, Ral, Rho, Rap, and Rab families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways.

Molecular Weight: 24 kDa

Gene ID: 9230

UniProt: Q15907

Pathways: Hormone Transport, Carbohydrate Homeostasis