EML4 antibody (AA 207-423)

Catalog No. ABIN6719384

The Rabbit Polyclonal anti-EML4 antibody is suitable to detect EML4 in samples from Human. It has been validated for WB, ELISA, IF, IHC and FACS.

Quick Overview for EML4 antibody (AA 207-423) (ABIN6719384)

Target: EML4 (Echinoderm Microtubule Associated Protein Like 4 (EML4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EML4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-EML4 Antibody

Binding Specificity: AA 207-423

Purpose: Anti-EML4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EML4 Antibody (ABIN6719384). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human EML4 recombinant protein (Position: K207-N423).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EML4

Target: EML4 (Echinoderm Microtubule Associated Protein Like 4 (EML4))

Alternative Name: EML4

Background:

Synonyms: Echinoderm microtubule-associated protein-like 4, EMAP-4, Restrictedly overexpressed proliferation-associated protein, Ropp 120, EML4, C2orf2, EMAPL4

Tissue Specificity: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.

Background: Echinoderm microtubule-associated protein-like 4 is a protein that in humans is encoded by the EML4gene. This gene is a member of the echinoderm microtubule associated protein-like family, and it mapped to 2p21. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants.

Molecular Weight: 120 kDa

Gene ID: 27436