KIF15 antibody (AA 1201-1388)

Catalog No. ABIN6719385

Product Details anti-KIF15 Antibody

Target: KIF15 (Kinesin Family Member 15 (KIF15))

Binding Specificity: AA 1201-1388

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF15 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for KIF15 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for KIF15 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KIF15 recombinant protein (Position: M1201-S1388).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for KIF15

Target: KIF15 (Kinesin Family Member 15 (KIF15))

Alternative Name: KIF15 (KIF15 Products)

Synonyms: XKlp2 antibody, kif15 antibody, klp2 antibody, KIF15 antibody, si:dkey-108k21.1 antibody, wu:fb96c12 antibody, wu:fc51g12 antibody, wu:fe01e02 antibody, HKLP2 antibody, KNSL7 antibody, NY-BR-62 antibody, 3110023M17Rik antibody, 3930402I10Rik antibody, D330038N01 antibody, Knsl7 antibody, b2b1117.1Clo antibody, kinesin family member 15 L homeolog antibody, kinesin family member 15 antibody, kinesin family member 15 S homeolog antibody, zinc finger protein 660 antibody, kif15.L antibody, KIF15 antibody, kif15.S antibody, kif15 antibody, Kif15 antibody, ZNF660 antibody

Background:

Synonyms: Kinesin-like protein KIF15, Kinesin-like protein 2, hKLP2, Kinesin-like protein 7, Serologically defined breast cancer antigen NY-BR-62, KIF15, KLP2, KNSL7

Background: Kinesin family member 15 is a protein that in humans is encoded by the KIF15 gene. It is mapped to 3p21.31. This gene encodes a motor protein that is part of the kinesin superfamily. KIF15 maintains half spindle separation by opposing forces generated by other motor proteins. KIF15 co-localizes with microtubules and actin filaments in both dividing cells and in postmitotic neurons. KIF15 (also known as Kinesin-12 and HKLP2) is a motor protein expressed in all cells during mitosis and in postmitotic neurons undergoing axon growth. KIF15 maintains bipolar microtubule spindle apparatus in dividing cells and shares redundant functions with KIF11. KIF15 is thought to promote spindle assembly by cross-linking and sliding along microtubules creating a separation between centrosomes. HeLa cells depleted of KIF11, with reduced microtubule dynamics, are able to form bipolar spindles from acentrosomal asters in a KIF15 dependent manner. Hence, inhibition of KIF15 function will be a vital therapeutic approach in cancer chemotherapy.

Gene ID: 56992