APEX1 antibody (AA 2-318) (DyLight 550)

Catalog No. ABIN6719389

This Mouse Monoclonal antibody specifically detects APEX1 in FACS. It exhibits reactivity toward Human.

Quick Overview for APEX1 antibody (AA 2-318) (DyLight 550) (ABIN6719389)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APEX1 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: 5C11

Product Details anti-APEX1 Antibody (DyLight 550)

Binding Specificity: AA 2-318

Purpose: Anti-Human APE1 DyLight® 550 conjugated APEX1 Antibody(monoclonal, 5C11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human APE1 DyLight® 550 conjugated APEX1 Antibody (monoclonal, 5C11) (ABIN5692978)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequence identity with mouse and rat APE1, respectively.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994. 2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3-prime mispaired DNA.Nature 415: 655-659, 2002. 3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res. 20: 4417-4421, 1992.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1

Background:

Synonyms: DNA- (apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA- (apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1

Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Molecular Weight: 39 kDa

Gene ID: 328

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus