HERC5 antibody (AA 793-1024)

Catalog No. ABIN6719405

The Rabbit Polyclonal anti-HERC5 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect HERC5 in samples from Human.

Quick Overview for HERC5 antibody (AA 793-1024) (ABIN6719405)

Target: HERC5 (Hect Domain and RLD 5 (HERC5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-HERC5 Antibody

Binding Specificity: AA 793-1024

Purpose: Anti-HERC5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HERC5 Antibody (ABIN6719405). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
ELISA, 0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HERC5

Target: HERC5 (Hect Domain and RLD 5 (HERC5))

Alternative Name: HERC5

Background:

Synonyms: E3 ISG15--protein ligase HERC5, Cyclin-E-binding protein 1, HECT domain and RCC1-like domain-containing protein 5, HERC5, CEB1, CEBP1

Tissue Specificity: Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Background: Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.

Molecular Weight: 117 kDa

Gene ID: 51191