HERC5 antibody (Middle Region)

Catalog No. ABIN2774739

This Rabbit Polyclonal antibody specifically detects HERC5 in WB and IHC. It exhibits reactivity toward Human, Cow, Rat, Horse, Rabbit and Pig.

Quick Overview for HERC5 antibody (Middle Region) (ABIN2774739)

Target: HERC5 (Hect Domain and RLD 5 (HERC5))

Reactivity: Human, Cow, Rat, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HERC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HERC5 Antibody

Binding Specificity: Middle Region

Purpose: HERC5 Antibody - middle region

Sequence: FHPEELKDVI VGNTDYDWKT FEKNARYEPG YNSSHPTIVM FWKAFHKLTL

Predicted Reactivity: Cow: 79%, Horse: 79%, Human: 100%, Pig: 79%, Rabbit: 86%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against HERC5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HERC5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43213-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HERC5

Target: HERC5 (Hect Domain and RLD 5 (HERC5))

Alternative Name: HERC5

Background:

Background Information: HERC5 is a member of the HERC family of ubiquitin ligases.It contains a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulates expression of HERC5 protein in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.

Gene Name: HECT and RLD domain containing E3 ubiquitin protein ligase 5

Alternative Symbols: CEB1, CEBP1

Protein Name: E3 ISG15--protein ligase HERC5

Molecular Weight: 117kDa

Gene ID: 51191

NCBI Accession: NP_057407

UniProt: Q69G20