NMI antibody (AA 2-307)
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- Target See all NMI Antibodies
- NMI (N-Myc (And STAT) Interactor (NMI))
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Binding Specificity
- AA 2-307
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Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NMI antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Mouse IgG monoclonal antibody for NMI detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
- Mouse IgG monoclonal antibody for NMI detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI.
- Clone
- 2F3
- Isotype
- IgG
- Top Product
- Discover our top product NMI Primary Antibody
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- Application Notes
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Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL
- Comment
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Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- NMI (N-Myc (And STAT) Interactor (NMI))
- Alternative Name
- NMI (NMI Products)
- Synonyms
- fj02e01 antibody, wu:fj02e01 antibody, NMI antibody, MGC108489 antibody, N-myc and STAT interactor antibody, N-myc (and STAT) interactor antibody, N-myc and STAT interactor L homeolog antibody, NMI antibody, Nmi antibody, nmi antibody, nmi.L antibody
- Background
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Synonyms: N-myc-interactor, Nmi, N-myc and STAT interactor, NMI
Background: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
- Gene ID
- 9111
- UniProt
- Q13287
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