IDE antibody (AA 485-756) (DyLight 550)

Catalog No. ABIN7354611

This Rabbit Polyclonal antibody specifically detects IDE in FACS. It exhibits reactivity toward Human.

Quick Overview for IDE antibody (AA 485-756) (DyLight 550) (ABIN7354611)

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDE antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Product Details anti-IDE Antibody (DyLight 550)

Binding Specificity: AA 485-756

Purpose: Anti-Human IDE DyLight® 550 conjugated Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human IDE DyLight® 550 conjugated Antibody -Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IDE recombinant protein (Position: F485-K756).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. "Entrez Gene: IDE insulin-degrading enzyme". 2. Affholter JA, Fried VA, Roth RA (Dec 1988). "Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III". Science. 242 (4884): 1415-8.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: IDE

Background:

Synonyms: Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE

Background: Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 39 kDa

Gene ID: 416

UniProt: P14735

Pathways: SARS-CoV-2 Protein Interactome