CD8 alpha antibody (AA 27-114)

Catalog No. ABIN6719524

This Rabbit Polyclonal antibody specifically detects CD8 alpha in IHC, WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for CD8 alpha antibody (AA 27-114) (ABIN6719524)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 27-114

Purpose: Anti-CD8 alpha/Cd8a Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD8 alpha/Cd8a Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived rat CD8 alpha/Cd8a recombinant protein (Position: Q27-Q114).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL ELISA, 0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: Cd8a

Background:

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Gene Full Name: CD8 subunit alpha

Molecular Weight: 26-35 kDa

Gene ID: 24930

UniProt: P07725

Pathways: TCR Signaling