Liver Arginase antibody (AA 25-183)

Catalog No. ABIN6719532

This anti-Liver Arginase antibody is a Rabbit Polyclonal antibody detecting Liver Arginase in WB, ELISA and IHC. Suitable for Human, Rat, Mouse and Monkey.

Quick Overview for Liver Arginase antibody (AA 25-183) (ABIN6719532)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Liver Arginase Antibody

Binding Specificity: AA 25-183

Purpose: Anti-liver Arginase/ARG1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-liver Arginase/ARG1 Antibody Picoband® (ABIN6719532). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X mutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. Mutat. 14: 355-356, 1999. 2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic study of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989. 3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and nucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: ARG1

Background:

Synonyms: Arginase-1, Liver-type arginase, Type I arginase, ARG1

Tissue Specificity: Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Background: ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98 % of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90 % of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50 % of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 37 kDa

Gene ID: 383

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin