CD2AP antibody (AA 253-337)

Catalog No. ABIN6719544

Product Details anti-CD2AP Antibody

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Binding Specificity: AA 253-337

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2AP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purpose: Mouse IgG monoclonal antibody for CD2AP detection. Tested with WB, IHC-P, FCM in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Mouse IgG monoclonal antibody for CD2AP detection. Tested with WB, IHC-P, FCM in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CD2AP recombinant protein (Position: K253-K337).

Clone: 5F8

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Flow Cytometry|1-3 μg/1x106 cells

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CD2AP

Target: CD2AP (Cd2ap) (CD2-Associated Protein (Cd2ap))

Alternative Name: CD2AP (Cd2ap Products)

Synonyms: cd2apl antibody, fj51c01 antibody, im:7153970 antibody, si:ch211-207n9.1 antibody, wu:fj51c01 antibody, zgc:103705 antibody, DKFZp469E061 antibody, CMS antibody, AL024079 antibody, C78928 antibody, METS-1 antibody, Mets1 antibody, CD2-associated protein L homeolog antibody, CD2 associated protein antibody, CD2-associated protein antibody, cd2ap.L antibody, CD2AP antibody, cd2ap antibody, Cd2ap antibody

Background:

Synonyms: CD2-associated protein, Adapter protein CMS, Cas ligand with multiple SH3 domains, CD2AP

Background: CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

Gene ID: 23607

UniProt: Q9Y5K6