LMAN1 antibody (AA 96-458)

Catalog No. ABIN6719563

This Rabbit Polyclonal antibody specifically detects LMAN1 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LMAN1 antibody (AA 96-458) (ABIN6719563)

Target: LMAN1 (Lectin, Mannose-Binding, 1 (LMAN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LMAN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-LMAN1 Antibody

Binding Specificity: AA 96-458

Purpose: Anti-LMAN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LMAN1 Antibody Picoband® (ABIN6719563). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human LMAN1 recombinant protein (Position: K96-M458).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Neve, E. P. A., Svensson, K., Fuxe, J., Petterson, R. F. VIPL, a VIP36-like membrane protein with a putative function in the export of glycoproteins from the endoplasmic reticulum. Exp. Cell Res. 288: 70-83, 2003. 2. Zhang, B., Spreafico, M., Zheng, C., Yang, A., Platzer, P., Callaghan, M. U., Avci, Z., Ozbek, N., Mahlangu, J., Haw, T., Kaufman, R. J., Marchant, K., Tuddenham, E. G. D., Seligsohn, U., Peyvandi, F., Ginsburg, D. Genotype-phenotype correlation in combined deficiency of factor V and factor VIII. Blood 111: 5592-5600, 2008.

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LMAN1

Target: LMAN1 (Lectin, Mannose-Binding, 1 (LMAN1))

Alternative Name: LMAN1

Background:

Synonyms: Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D

Tissue Specificity: Ubiquitous.

Background: Protein ERGIC-53 also known as ER-Golgi intermediate compartment 53 kDa protein or lectin mannose-binding 1 is a protein that in humans is encoded by the LMAN1 gene. It is mapped to 18q21.32. The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH -sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.

Molecular Weight: 58 kDa

Gene ID: 3998

UniProt: P49257