FRZB antibody (AA 49-311)

Catalog No. ABIN6719567

The Rabbit Polyclonal anti-FRZB antibody is suitable to detect FRZB in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for FRZB antibody (AA 49-311) (ABIN6719567)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FRZB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FRZB Antibody

Binding Specificity: AA 49-311

Purpose: Anti-FRZB Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FRZB Antibody (ABIN6719567). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse FRZB recombinant protein (Position: N49-K311).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: Frzb

Background:

Synonyms: Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, Frzb, Fiz, Fre, Frzb1, Sfrp3

Tissue Specificity: Expressed in kidney, brain, testis. Weak expression in spleen and heart.

Background: FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Molecular Weight: 36 kDa

Gene ID: 20378

UniProt: P97401

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation