FRZB antibody (AA 49-311)

Catalog No. ABIN6719567

The Rabbit Polyclonal anti-FRZB antibody has been validated for WB and ELISA. It is suitable to detect FRZB in samples from Human, Mouse and Rat.

Quick Overview for FRZB antibody (AA 49-311) (ABIN6719567)

Target: FRZB (Frizzled-Related Protein (FRZB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FRZB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FRZB Antibody

Binding Specificity: AA 49-311

Purpose: Anti-FRZB Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FRZB Antibody Picoband® (ABIN6719567). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse FRZB recombinant protein (Position: N49-K311).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Baker-Lepain JC, et al. Variant alleles of the Wnt antagonist FRZB are determinants of hip shape and modify the relationship between hip shape and osteoarthritis. Arthritis Rheum, 2012 May. 2. Qin S, et al. FRZB knockdown upregulates β-catenin activity and enhances cell aggressiveness in gastric cancer. Oncol Rep, 2014 May.  3. Wasmann RA, et al. Novel membrane frizzled-related protein gene mutation as cause of posterior microphthalmia resulting in high hyperopia with macular folds. Acta Ophthalmol, 2014 May.

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: Frzb

Background:

Synonyms: Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, Frzb, Fiz, Fre, Frzb1, Sfrp3

Tissue Specificity: Expressed in kidney, brain, testis. Weak expression in spleen and heart.

Background: FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Molecular Weight: 36 kDa

Gene ID: 20378

UniProt: P97401

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation