Grancalcin antibody (AA 42-217)

Catalog No. ABIN6719568

Product Details anti-Grancalcin Antibody

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Binding Specificity: AA 42-217

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Grancalcin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit IgG polyclonal antibody for Grancalcin/GCA detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Grancalcin/GCA detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Grancalcin/GCA recombinant protein (Position: A42-I217).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Grancalcin

Target: Grancalcin (GCA) (Grancalcin, EF-Hand Calcium Binding Protein (GCA))

Alternative Name: GCA (GCA Products)

Synonyms: Grancalcin antibody, 5133401E04Rik antibody, AI573844 antibody, GCL antibody, zgc:92027 antibody, grancalcin antibody, Grancalcin antibody, zgc:92027 antibody, GCA antibody, gran antibody, LOC100280890 antibody, Gca antibody

Background:

Synonyms: Grancalcin, GCA, GCL

Background: Grancalcin is a protein that in humans is encoded by the GCA gene. This gene is mapped to 2q24.2. This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction, with magnesium alone, it partitions with the granule fraction, and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.

Gene ID: 25801

UniProt: P28676