GOLPH3 antibody (AA 56-298)

Catalog No. ABIN6719570

Product Details anti-GOLPH3 Antibody

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Binding Specificity: AA 56-298

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOLPH3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for GOLPH3 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for GOLPH3 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human GOLPH3 recombinant protein (Position: K56-K298).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunohistochemistry(Frozen Section)|0.5-1 μg/mL Immunocytochemistry/Immunofluorescence|2 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for GOLPH3

Target: GOLPH3 (Golgi phosphoprotein 3 (Coat-Protein) (GOLPH3))

Alternative Name: GOLPH3 (GOLPH3 Products)

Synonyms: wu:fj66a10 antibody, zgc:101573 antibody, zgc:110223 antibody, 4733401N08Rik antibody, 5730410D03Rik antibody, AW413496 antibody, GOPP1 antibody, GPP34 antibody, MIDAS antibody, Gmx33 antibody, golgi phosphoprotein 3 antibody, golph3 antibody, CpipJ_CPIJ009278 antibody, Golph3 antibody, GOLPH3 antibody

Background:

Synonyms: Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS, GOLPH3, GPP34

Background: Golgi phosphoprotein 3 (GOLPH3) is a human protein encoded by GOLPH3 gene. It is mapped to 5p13.3. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Gene ID: 64083

UniProt: Q9H4A6